The present study aimed to evaluate the antimicrobial activity of essential oils of the 2 thymus (Thymus vulgaris L. and Th. kotschyanus Boiss. et Hohen.) species with antimicrobial potential of four antibiotics (gentamicin, penicilin, nalidixic acid and amoxicillin). The selected essential oils were screened against Gram (-) bacteria (Escherichia coli) and Gram (+) bacteria (Staphylococcus aureus) using disc diffusion method. According to results of our study of the bacterial inhibition zone diameter these oils lack any antibacterial effect.