Xəbərlər AMEA Biologiya və tibb elmləri bölməsinin rəsmi jurnalı
Enzymes Involved In The Detoxification Of H2O2 In The Leaves And Roots Of Wheat Subjected To Long-Term Soil Drought
I.M.Huseynova, D.R.Aliyeva, A.Ch.Mammadov, J.A. Aliyev

Institute of Botany, ANAS
The dynamics of the activity of catalase, ascorbate peroxidase, guaiacol peroxidase and benzidine peroxi-dase, as well as the level of hydrogen peroxide in the vegetative organs of durum wheat (Triticum durum Desf.) cultivars was studied under long-term soil drought conditions. It was established that hydrogen perox-ide generation occurred at early stages of stress in the tolerant variety Barakatli-95, whereas in the suscepti-ble variety Garagylchyg-2 its significant amounts were accumulated only at later stages. The highest activity of catalase which plays a leading role in the neutralization of hydrogen peroxide was observed in the leaves and roots of the drought tolerant variety Barakatli-95. Despite the fact that the protection system also in-cludes peroxidases, the activity of these enzymes even after synthesis of their new portions is substantially lower compared with catalase. Native PAGE electrophoresis revealed the presence of one isoform of CAT, seven isoforms of APX, three isoforms of GPO and three isoforms of BPO in the leaves, and also three isoforms of CAT, four isoforms of APX, two isoforms of GPO and six isoforms of BPO in the roots of wheat. One isoform of CAT was found in the roots when water supply is normal and three isoforms were ob-served under drought conditions. Stress associated with long-term soil drought in the roots of wheat has led to an increase in the heterogeneity due to the formation of two new sedentary forms of catalase: CAT2 and CAT3.

Triticum durum Desf., hydrogen peroxide, peroxidase, catalase, drought stress 15