Xəbərlər AMEA Biologiya və tibb elmləri bölməsinin rəsmi jurnalı
İdentification Of Sr11, Sr26 and Sr31 Genes Effective Resistant To Stem Rust Rase TKTTF in Wheat Genotypes in Azerbaijan
S.M. Rustamova, A.T. Haciyeva, Sh.F. Sadigov, A.Ch. Mammadov, I.M. Huseynova

Institute of Molecular Biology and Biotechnologies, Azerbaijan National Academy of Sciences 

 

For the first time wheat germplasm has been examined and gene sources effective resistant to TKTTF ras group of stem rust spread in Azerbaijan have been characterized using KASP (Kompetitive Allele Specific PCR) genotyping technology. Real-time PCR (RT-RCR) analysis with diallel-type KASP_6BL_BS0074288_51 primer identified Sr11gene on 6B chromosome of 9 wheat genotypes from 34 ones, and it was identified in 7 genotypes from 10 ones when using KASp_6BL_Tdurum contig55744_822. A fragment of 207bp, which is considered to be  the diagnostic amplicon for theSr26 gene has been synthesized in 11 wheat genotypes from 42 ones as a result of PCR performed with a dominant STS marker Sr26#43. Lr26-Sr31-Yr9 locus located in  1BL.1RS translocation of 42 wheat genotypes was analyzed using specific Iag95 primer and 1100 bp PCR products, characteristic of Sr31 gene  were found to be synthesized in 8 genotypes.

 

Wheat, Puccinia graminis f. sp. tritici, Sr11, Sr26, Sr31, KASP-genotyping, RT-PZR