Xəbərlər AMEA Biologiya və tibb elmləri bölməsinin rəsmi jurnalı
Evaluation of Genetic Diversity in Bread Wheat ( T.aestivum L.) Using ISSR Markers
S. Nuriyeva1, Z. Akparov1, M. Abbasov1,2, Kh. Rustamov1, H. Sadigov1, J. Ocaqi1, F. Sheykhzamanova1, S. Rzaeva1, R. Sharma3

1 Institute of Genetic Resources, ANAS
2Baku State University
3 International Center for Agricultural Research in the Dry Areas (ICARDA),
Tashkent, Uzbekistan
Genetic diversity of 50 bread wheat accessions belonging to 6 botanical varieties was studied using ISSR
primers. Only 7 ISSR primers out of 12 which showed a high level of polymorphism were used for the
genetic diversity analysis of the studied genotypes. Sixty five polymorphic fragments were identified with
these primers and cluster analyses divided all genotypes into 5 main clusters according to the Nei’s similarity
index. The results of cluster analysis showed that the genotypes of the same botanical varieties generally
were included into one group. This once again confirms the importance of botanical variety traits in
breeding. Genetically distinct genotypes can be used in breeding as an initial material. The primers UBC-
811, UBC-841 and UBC-827 with the highest number of amplified and polymorphic bands, the level of
polymorphism and the highest value of genetic diversity, such as PIC, EMR and RP were recommended as
the most appropriate primers to study genetic structure of bread wheat genotypes.

T. aestivum L., genetic diversity, botanical vaieties, ISSR marker